Construct: shRNA TRCN0000196478

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004635.3-1983s1c1
DNA Barcode:
GAACTTAAGGTGGGAGATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPKAPK3 (7867)
Gene Description:
MAPK activated protein kinase 3
Transcript:
RefSeq NM_004635.3 (NON-CURRENT)
Match location:
Position 1983 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196478)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACTTAAGGTGGGAGATATT
Hairpin Sequence:
5'-CCGG-GAACTTAAGGTGGGAGATATT-CTCGAG-AATATCTCCCACCTTAAGTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACTTAAGGTGGGAGATATTCTCGAGAATATCTCCCACCTTAAGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACTTAAGGTGGGAGATATTCTCGAGAATATCTCCCACCTTAAGTTC-3'

Other clones with same target sequence:

(none)