Construct: shRNA TRCN0000196493

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022048.3-2543s1c1
DNA Barcode:
GCATATTGTGTCTGGTTATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSNK1G1 (53944)
Gene Description:
casein kinase 1 gamma 1
Transcript:
RefSeq NM_022048.3 (NON-CURRENT)
Match location:
Position 2543 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196493)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATATTGTGTCTGGTTATAT
Hairpin Sequence:
5'-CCGG-GCATATTGTGTCTGGTTATAT-CTCGAG-ATATAACCAGACACAATATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATATTGTGTCTGGTTATATCTCGAGATATAACCAGACACAATATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATATTGTGTCTGGTTATATCTCGAGATATAACCAGACACAATATGC-3'

Other clones with same target sequence:

(none)