Construct: shRNA TRCN0000196522

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006180.3-5188s1c1
DNA Barcode:
GCTATTTGAATGGTCAGATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NTRK2 (4915)
Gene Description:
neurotrophic receptor tyrosine kinase 2
Transcript:
RefSeq NM_006180.3 (NON-CURRENT)
Match location:
Position 5188 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196522)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATTTGAATGGTCAGATAT
Hairpin Sequence:
5'-CCGG-GCTATTTGAATGGTCAGATAT-CTCGAG-ATATCTGACCATTCAAATAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATTTGAATGGTCAGATATCTCGAGATATCTGACCATTCAAATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATTTGAATGGTCAGATATCTCGAGATATCTGACCATTCAAATAGC-3'

Other clones with same target sequence:

  1. TRCN0000273090