Construct: shRNA TRCN0000196590

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001527.1-789s1c1
DNA Barcode:
GACGGTATCATTCCATAAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HDAC2 (3066)
Gene Description:
histone deacetylase 2
Transcript:
RefSeq NM_001527.1 (NON-CURRENT)
Match location:
Position 789 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196590)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACGGTATCATTCCATAAATA
Hairpin Sequence:
5'-CCGG-GACGGTATCATTCCATAAATA-CTCGAG-TATTTATGGAATGATACCGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACGGTATCATTCCATAAATACTCGAGTATTTATGGAATGATACCGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACGGTATCATTCCATAAATACTCGAGTATTTATGGAATGATACCGTC-3'

Other clones with same target sequence:

  1. TRCN0000342551