Construct: shRNA TRCN0000196598

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144610.1-1207s1c1
DNA Barcode:
GTACTGATACTAGACCATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RSKR (124923)
Gene Description:
ribosomal protein S6 kinase related
Transcript:
RefSeq NM_144610.1 (NON-CURRENT)
Match location:
Position 1207 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196598)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACTGATACTAGACCATTTA
Hairpin Sequence:
5'-CCGG-GTACTGATACTAGACCATTTA-CTCGAG-TAAATGGTCTAGTATCAGTAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACTGATACTAGACCATTTACTCGAGTAAATGGTCTAGTATCAGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACTGATACTAGACCATTTACTCGAGTAAATGGTCTAGTATCAGTAC-3'

Other clones with same target sequence:

(none)