Construct: shRNA TRCN0000196620

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003318.3-1821s1c1
DNA Barcode:
GATAAGATCATCCGACTTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TTK (7272)
Gene Description:
TTK protein kinase
Transcript:
RefSeq NM_003318.3 (NON-CURRENT)
Match location:
Position 1821 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196620)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATAAGATCATCCGACTTTAT
Hairpin Sequence:
5'-CCGG-GATAAGATCATCCGACTTTAT-CTCGAG-ATAAAGTCGGATGATCTTATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATAAGATCATCCGACTTTATCTCGAGATAAAGTCGGATGATCTTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATAAGATCATCCGACTTTATCTCGAGATAAAGTCGGATGATCTTATC-3'

Other clones with same target sequence:

  1. TRCN0000342614