Construct: shRNA TRCN0000196655

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004958.2-8245s1c1
DNA Barcode:
GCAGAAATGTTTGCAAGATAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MTOR (2475)
Gene Description:
mechanistic target of rapamycin kinase
Transcript:
RefSeq NM_004958.2 (NON-CURRENT)
Match location:
Position 8245 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196655)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAAATGTTTGCAAGATAG
Hairpin Sequence:
5'-CCGG-GCAGAAATGTTTGCAAGATAG-CTCGAG-CTATCTTGCAAACATTTCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAAATGTTTGCAAGATAGCTCGAGCTATCTTGCAAACATTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAAATGTTTGCAAGATAGCTCGAGCTATCTTGCAAACATTTCTGC-3'

Other clones with same target sequence:

(none)