Construct: shRNA TRCN0000196661

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000051.3-6606s1c1
DNA Barcode:
GCCATATTCTTTCCGTCTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATM (472)
Gene Description:
ATM serine/threonine kinase
Transcript:
RefSeq NM_000051.3 (CURRENT)
Match location:
Position 6606 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196661)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATATTCTTTCCGTCTATT
Hairpin Sequence:
5'-CCGG-GCCATATTCTTTCCGTCTATT-CTCGAG-AATAGACGGAAAGAATATGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATATTCTTTCCGTCTATTCTCGAGAATAGACGGAAAGAATATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATATTCTTTCCGTCTATTCTCGAGAATAGACGGAAAGAATATGGC-3'

Other clones with same target sequence:

(none)