Construct: shRNA TRCN0000196700

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000167.3-2559s1c1
DNA Barcode:
GCAACGGTTATGCATAATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GK (2710)
Gene Description:
glycerol kinase
Transcript:
RefSeq NM_000167.3 (NON-CURRENT)
Match location:
Position 2559 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196700)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACGGTTATGCATAATATT
Hairpin Sequence:
5'-CCGG-GCAACGGTTATGCATAATATT-CTCGAG-AATATTATGCATAACCGTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACGGTTATGCATAATATTCTCGAGAATATTATGCATAACCGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACGGTTATGCATAATATTCTCGAGAATATTATGCATAACCGTTGC-3'

Other clones with same target sequence:

  1. TRCN0000296846