Construct: shRNA TRCN0000196723

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_370878.3-7399s1c1
DNA Barcode:
GCATAGAACATGTGCACATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PEAK1 (79834)
Gene Description:
pseudopodium enriched atypical kinase 1
Transcript:
RefSeq XM_370878.3 (NON-CURRENT)
Match location:
Position 7399 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196723)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATAGAACATGTGCACATAA
Hairpin Sequence:
5'-CCGG-GCATAGAACATGTGCACATAA-CTCGAG-TTATGTGCACATGTTCTATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATAGAACATGTGCACATAACTCGAGTTATGTGCACATGTTCTATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATAGAACATGTGCACATAACTCGAGTTATGTGCACATGTTCTATGC-3'

Other clones with same target sequence:

(none)