Construct: shRNA TRCN0000196793

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004850.3-583s1c1
DNA Barcode:
GCTTGCTGGATGGCTTAAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ROCK2 (9475)
Gene Description:
Rho associated coiled-coil containing protein kinase 2
Transcript:
RefSeq NM_004850.3 (NON-CURRENT)
Match location:
Position 583 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196793)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGCTGGATGGCTTAAATT
Hairpin Sequence:
5'-CCGG-GCTTGCTGGATGGCTTAAATT-CTCGAG-AATTTAAGCCATCCAGCAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGCTGGATGGCTTAAATTCTCGAGAATTTAAGCCATCCAGCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGCTGGATGGCTTAAATTCTCGAGAATTTAAGCCATCCAGCAAGC-3'

Other clones with same target sequence:

  1. TRCN0000342473