Construct: shRNA TRCN0000196823

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_042066.11-4658s1c1
DNA Barcode:
GCAATTACAATCTCTTCATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K1 (4214)
Gene Description:
mitogen-activated protein kinase kinase kinase 1
Transcript:
RefSeq XM_042066.11 (NON-CURRENT)
Match location:
Position 4658 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196823)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATTACAATCTCTTCATTG
Hairpin Sequence:
5'-CCGG-GCAATTACAATCTCTTCATTG-CTCGAG-CAATGAAGAGATTGTAATTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATTACAATCTCTTCATTGCTCGAGCAATGAAGAGATTGTAATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATTACAATCTCTTCATTGCTCGAGCAATGAAGAGATTGTAATTGC-3'

Other clones with same target sequence:

(none)