Construct: shRNA TRCN0000196831

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006251.5-4192s1c1
DNA Barcode:
GCACAGACAATTGCAGTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKAA1 (5562)
Gene Description:
protein kinase AMP-activated catalytic subunit alpha 1
Transcript:
RefSeq NM_006251.5 (CURRENT)
Match location:
Position 4192 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196831)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAGACAATTGCAGTAAAT
Hairpin Sequence:
5'-CCGG-GCACAGACAATTGCAGTAAAT-CTCGAG-ATTTACTGCAATTGTCTGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAGACAATTGCAGTAAATCTCGAGATTTACTGCAATTGTCTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAGACAATTGCAGTAAATCTCGAGATTTACTGCAATTGTCTGTGC-3'

Other clones with same target sequence:

(none)