Construct: shRNA TRCN0000196880

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007271.2-503s1c1
DNA Barcode:
GAAACAGAGTTTCTTCGTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK38 (11329)
Gene Description:
serine/threonine kinase 38
Transcript:
RefSeq NM_007271.2 (NON-CURRENT)
Match location:
Position 503 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196880)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAACAGAGTTTCTTCGTTTG
Hairpin Sequence:
5'-CCGG-GAAACAGAGTTTCTTCGTTTG-CTCGAG-CAAACGAAGAAACTCTGTTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAACAGAGTTTCTTCGTTTGCTCGAGCAAACGAAGAAACTCTGTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAACAGAGTTTCTTCGTTTGCTCGAGCAAACGAAGAAACTCTGTTTC-3'

Other clones with same target sequence:

(none)