Construct: shRNA TRCN0000196887

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_170693.1-1702s1c1
DNA Barcode:
GTTCTGACACTAAGACATTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SGK2 (10110)
Gene Description:
serum/glucocorticoid regulated kinase 2
Transcript:
RefSeq NM_170693.1 (NON-CURRENT)
Match location:
Position 1702 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196887)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCTGACACTAAGACATTAG
Hairpin Sequence:
5'-CCGG-GTTCTGACACTAAGACATTAG-CTCGAG-CTAATGTCTTAGTGTCAGAAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCTGACACTAAGACATTAGCTCGAGCTAATGTCTTAGTGTCAGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCTGACACTAAGACATTAGCTCGAGCTAATGTCTTAGTGTCAGAAC-3'

Other clones with same target sequence:

(none)