Construct: shRNA TRCN0000196897

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004384.3-1356s1c1
DNA Barcode:
GCTAGATATATGAGCATAAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSNK1G3 (1456)
Gene Description:
casein kinase 1 gamma 3
Transcript:
RefSeq NM_004384.3 (NON-CURRENT)
Match location:
Position 1356 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196897)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAGATATATGAGCATAAAC
Hairpin Sequence:
5'-CCGG-GCTAGATATATGAGCATAAAC-CTCGAG-GTTTATGCTCATATATCTAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAGATATATGAGCATAAACCTCGAGGTTTATGCTCATATATCTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAGATATATGAGCATAAACCTCGAGGTTTATGCTCATATATCTAGC-3'

Other clones with same target sequence:

(none)