Construct: shRNA TRCN0000196922

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005922.2-3031s1c1
DNA Barcode:
GCAGTCCATTGAGGGACTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K4 (4216)
Gene Description:
mitogen-activated protein kinase kinase kinase 4
Transcript:
RefSeq NM_005922.2 (NON-CURRENT)
Match location:
Position 3031 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196922)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTCCATTGAGGGACTTAT
Hairpin Sequence:
5'-CCGG-GCAGTCCATTGAGGGACTTAT-CTCGAG-ATAAGTCCCTCAATGGACTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTCCATTGAGGGACTTATCTCGAGATAAGTCCCTCAATGGACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTCCATTGAGGGACTTATCTCGAGATAAGTCCCTCAATGGACTGC-3'

Other clones with same target sequence:

(none)