Construct: shRNA TRCN0000196924

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001278.3-2753s1c1
DNA Barcode:
GCTGCTCACAAGTTCTATTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHUK (1147)
Gene Description:
component of inhibitor of nuclear factor kappa B kinase complex
Transcript:
RefSeq NM_001278.3 (NON-CURRENT)
Match location:
Position 2753 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196924)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTCACAAGTTCTATTTC
Hairpin Sequence:
5'-CCGG-GCTGCTCACAAGTTCTATTTC-CTCGAG-GAAATAGAACTTGTGAGCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTCACAAGTTCTATTTCCTCGAGGAAATAGAACTTGTGAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTCACAAGTTCTATTTCCTCGAGGAAATAGAACTTGTGAGCAGC-3'

Other clones with same target sequence:

(none)