Construct: shRNA TRCN0000196936

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005028.3-831s1c1
DNA Barcode:
GCCACCGTTTGTCTGTGTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIP4K2A (5305)
Gene Description:
phosphatidylinositol-5-phosphate 4-kinase type 2 alpha
Transcript:
RefSeq NM_005028.3 (NON-CURRENT)
Match location:
Position 831 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196936)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACCGTTTGTCTGTGTATA
Hairpin Sequence:
5'-CCGG-GCCACCGTTTGTCTGTGTATA-CTCGAG-TATACACAGACAAACGGTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACCGTTTGTCTGTGTATACTCGAGTATACACAGACAAACGGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACCGTTTGTCTGTGTATACTCGAGTATACACAGACAAACGGTGGC-3'

Other clones with same target sequence:

(none)