Construct: shRNA TRCN0000196996

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003010.2-824s1c1
DNA Barcode:
GACAGCTTGTGGACTCTATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP2K4 (6416)
Gene Description:
mitogen-activated protein kinase kinase 4
Transcript:
RefSeq NM_003010.2 (NON-CURRENT)
Match location:
Position 824 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196996)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACAGCTTGTGGACTCTATTG
Hairpin Sequence:
5'-CCGG-GACAGCTTGTGGACTCTATTG-CTCGAG-CAATAGAGTCCACAAGCTGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACAGCTTGTGGACTCTATTGCTCGAGCAATAGAGTCCACAAGCTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACAGCTTGTGGACTCTATTGCTCGAGCAATAGAGTCCACAAGCTGTC-3'

Other clones with same target sequence:

(none)