Construct: shRNA TRCN0000197072

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020168.3-3092s1c1
DNA Barcode:
GCTCTCACTTTACAGAATGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAK6 (56924)
Gene Description:
p21 (RAC1) activated kinase 6
Transcript:
RefSeq NM_020168.3 (NON-CURRENT)
Match location:
Position 3092 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197072)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTCACTTTACAGAATGGA
Hairpin Sequence:
5'-CCGG-GCTCTCACTTTACAGAATGGA-CTCGAG-TCCATTCTGTAAAGTGAGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTCACTTTACAGAATGGACTCGAGTCCATTCTGTAAAGTGAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTCACTTTACAGAATGGACTCGAGTCCATTCTGTAAAGTGAGAGC-3'

Other clones with same target sequence:

(none)