Construct: shRNA TRCN0000197077

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002757.2-701s1c1
DNA Barcode:
GCTGGTAATTCGCATCAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP2K5 (5607)
Gene Description:
mitogen-activated protein kinase kinase 5
Transcript:
RefSeq NM_002757.2 (NON-CURRENT)
Match location:
Position 701 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197077)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGTAATTCGCATCAAGAT
Hairpin Sequence:
5'-CCGG-GCTGGTAATTCGCATCAAGAT-CTCGAG-ATCTTGATGCGAATTACCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGTAATTCGCATCAAGATCTCGAGATCTTGATGCGAATTACCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGTAATTCGCATCAAGATCTCGAGATCTTGATGCGAATTACCAGC-3'

Other clones with same target sequence:

  1. TRCN0000320675