Construct: shRNA TRCN0000197135

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003558.1-1736s1c1
DNA Barcode:
GCTGACTGAAGGACAAAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIP5K1B (8395)
Gene Description:
phosphatidylinositol-4-phosphate 5-kinase type 1 beta
Transcript:
RefSeq NM_003558.1 (NON-CURRENT)
Match location:
Position 1736 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197135)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGACTGAAGGACAAAGTTT
Hairpin Sequence:
5'-CCGG-GCTGACTGAAGGACAAAGTTT-CTCGAG-AAACTTTGTCCTTCAGTCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGACTGAAGGACAAAGTTTCTCGAGAAACTTTGTCCTTCAGTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGACTGAAGGACAAAGTTTCTCGAGAAACTTTGTCCTTCAGTCAGC-3'

Other clones with same target sequence:

(none)