Construct: shRNA TRCN0000197145

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002356.4-474s1c1
DNA Barcode:
GAATGGCCACGTGAAGGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MARCKS (4082)
Gene Description:
myristoylated alanine rich protein kinase C substrate
Transcript:
RefSeq NM_002356.4 (NON-CURRENT)
Match location:
Position 474 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197145)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATGGCCACGTGAAGGTAAA
Hairpin Sequence:
5'-CCGG-GAATGGCCACGTGAAGGTAAA-CTCGAG-TTTACCTTCACGTGGCCATTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATGGCCACGTGAAGGTAAACTCGAGTTTACCTTCACGTGGCCATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATGGCCACGTGAAGGTAAACTCGAGTTTACCTTCACGTGGCCATTC-3'

Other clones with same target sequence:

(none)