Construct: shRNA TRCN0000197398

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025819.2-2688s1c1
DNA Barcode:
CGGGAACTTTATGATTGTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Swt1 (66875)
Gene Description:
SWT1 RNA endoribonuclease homolog (S. cerevisiae)
Transcript:
RefSeq NM_025819.2 (NON-CURRENT)
Match location:
Position 2688 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197398)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGAACTTTATGATTGTGTT
Hairpin Sequence:
5'-CCGG-CGGGAACTTTATGATTGTGTT-CTCGAG-AACACAATCATAAAGTTCCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGAACTTTATGATTGTGTTCTCGAGAACACAATCATAAAGTTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGAACTTTATGATTGTGTTCTCGAGAACACAATCATAAAGTTCCCG-3'

Other clones with same target sequence:

(none)