Construct: shRNA TRCN0000197406

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153513.1-371s1c1
DNA Barcode:
CAAATCAGAATGATGCCATGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
BC028528 (229600)
Gene Description:
cDNA sequence BC028528
Transcript:
RefSeq NM_153513.1 (NON-CURRENT)
Match location:
Position 371 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197406)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAATCAGAATGATGCCATGT
Hairpin Sequence:
5'-CCGG-CAAATCAGAATGATGCCATGT-CTCGAG-ACATGGCATCATTCTGATTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAATCAGAATGATGCCATGTCTCGAGACATGGCATCATTCTGATTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAATCAGAATGATGCCATGTCTCGAGACATGGCATCATTCTGATTTG-3'

Other clones with same target sequence:

(none)