Construct: shRNA TRCN0000197462

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153125.1-712s1c1
DNA Barcode:
CCTAACTTCCAGGTGTTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sec16a (227648)
Gene Description:
SEC16 homolog A, endoplasmic reticulum export factor
Transcript:
RefSeq NM_153125.1 (NON-CURRENT)
Match location:
Position 712 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197462)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAACTTCCAGGTGTTTAAA
Hairpin Sequence:
5'-CCGG-CCTAACTTCCAGGTGTTTAAA-CTCGAG-TTTAAACACCTGGAAGTTAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAACTTCCAGGTGTTTAAACTCGAGTTTAAACACCTGGAAGTTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAACTTCCAGGTGTTTAAACTCGAGTTTAAACACCTGGAAGTTAGG-3'

Other clones with same target sequence:

  1. TRCN0000277109