Construct: shRNA TRCN0000197497

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198603.1-2371s1c1
DNA Barcode:
CCATGCCTATTATGATGCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Efcab12 (212516)
Gene Description:
EF-hand calcium binding domain 12
Transcript:
RefSeq NM_198603.1 (NON-CURRENT)
Match location:
Position 2371 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197497)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGCCTATTATGATGCAAA
Hairpin Sequence:
5'-CCGG-CCATGCCTATTATGATGCAAA-CTCGAG-TTTGCATCATAATAGGCATGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGCCTATTATGATGCAAACTCGAGTTTGCATCATAATAGGCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGCCTATTATGATGCAAACTCGAGTTTGCATCATAATAGGCATGG-3'

Other clones with same target sequence:

(none)