Construct: shRNA TRCN0000197505

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027482.1-513s1c1
DNA Barcode:
CAGACATTTGATAGCCATGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
5730508B09Rik (70617)
Gene Description:
RIKEN cDNA 5730508B09 gene
Transcript:
RefSeq NM_027482.1 (NON-CURRENT)
Match location:
Position 513 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197505)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGACATTTGATAGCCATGTA
Hairpin Sequence:
5'-CCGG-CAGACATTTGATAGCCATGTA-CTCGAG-TACATGGCTATCAAATGTCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGACATTTGATAGCCATGTACTCGAGTACATGGCTATCAAATGTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGACATTTGATAGCCATGTACTCGAGTACATGGCTATCAAATGTCTG-3'

Other clones with same target sequence:

(none)