Construct: shRNA TRCN0000197523

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026221.1-4029s1c1
DNA Barcode:
CCTTTGCTAAACTCATCATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppfibp1 (67533)
Gene Description:
PTPRF interacting protein, binding protein 1 (liprin beta 1)
Transcript:
RefSeq NM_026221.1 (NON-CURRENT)
Match location:
Position 4029 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197523)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTGCTAAACTCATCATTA
Hairpin Sequence:
5'-CCGG-CCTTTGCTAAACTCATCATTA-CTCGAG-TAATGATGAGTTTAGCAAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTGCTAAACTCATCATTACTCGAGTAATGATGAGTTTAGCAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTGCTAAACTCATCATTACTCGAGTAATGATGAGTTTAGCAAAGG-3'

Other clones with same target sequence:

(none)