Construct: shRNA TRCN0000197541

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172987.2-2267s1c1
DNA Barcode:
CCTGGCAAATTCATTAACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nkain3 (269513)
Gene Description:
Na+/K+ transporting ATPase interacting 3
Transcript:
RefSeq NM_172987.2 (CURRENT)
Match location:
Position 2267 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197541)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGCAAATTCATTAACAAA
Hairpin Sequence:
5'-CCGG-CCTGGCAAATTCATTAACAAA-CTCGAG-TTTGTTAATGAATTTGCCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGCAAATTCATTAACAAACTCGAGTTTGTTAATGAATTTGCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGCAAATTCATTAACAAACTCGAGTTTGTTAATGAATTTGCCAGG-3'

Other clones with same target sequence:

(none)