Construct: shRNA TRCN0000197587

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175465.2-2009s1c1
DNA Barcode:
CGACGGAACTGAACAATATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sestd1 (228071)
Gene Description:
SEC14 and spectrin domains 1
Transcript:
RefSeq NM_175465.2 (NON-CURRENT)
Match location:
Position 2009 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197587)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACGGAACTGAACAATATTT
Hairpin Sequence:
5'-CCGG-CGACGGAACTGAACAATATTT-CTCGAG-AAATATTGTTCAGTTCCGTCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACGGAACTGAACAATATTTCTCGAGAAATATTGTTCAGTTCCGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACGGAACTGAACAATATTTCTCGAGAAATATTGTTCAGTTCCGTCG-3'

Other clones with same target sequence:

(none)