Construct: shRNA TRCN0000197604
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_001029983.1-2980s1c1
- DNA Barcode:
- CCTGTCTTTGATGAGTTGTTA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Man1b1 (227619)
- Gene Description:
- mannosidase, alpha, class 1B, member 1
- Transcript:
- RefSeq NM_001029983.1 (NON-CURRENT)
- Match location:
- Position 2980 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000197604)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCTGTCTTTGATGAGTTGTTA
- Hairpin Sequence:
-
5'-CCGG-CCTGTCTTTGATGAGTTGTTA-CTCGAG-TAACAACTCATCAAAGACAGG-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCTGTCTTTGATGAGTTGTTACTCGAGTAACAACTCATCAAAGACAGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCTGTCTTTGATGAGTTGTTACTCGAGTAACAACTCATCAAAGACAGG-3'
Other clones with same target sequence:
(none)
