Construct: shRNA TRCN0000197620

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178612.2-362s1c1
DNA Barcode:
CTGGATTACAATGTTCATGCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cnpy4 (66455)
Gene Description:
canopy FGF signaling regulator 4
Transcript:
RefSeq NM_178612.2 (NON-CURRENT)
Match location:
Position 362 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197620)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGATTACAATGTTCATGCT
Hairpin Sequence:
5'-CCGG-CTGGATTACAATGTTCATGCT-CTCGAG-AGCATGAACATTGTAATCCAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGATTACAATGTTCATGCTCTCGAGAGCATGAACATTGTAATCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGATTACAATGTTCATGCTCTCGAGAGCATGAACATTGTAATCCAG-3'

Other clones with same target sequence:

(none)