Construct: shRNA TRCN0000197641

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001031664.1-1032s1c1
DNA Barcode:
CCCTTCTAATATCTCTAGCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nudt10 (102954)
Gene Description:
nudix (nucleoside diphosphate linked moiety X)-type motif 10
Transcript:
RefSeq NM_001031664.1 (CURRENT)
Match location:
Position 1032 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197641)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTCTAATATCTCTAGCAT
Hairpin Sequence:
5'-CCGG-CCCTTCTAATATCTCTAGCAT-CTCGAG-ATGCTAGAGATATTAGAAGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTCTAATATCTCTAGCATCTCGAGATGCTAGAGATATTAGAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTCTAATATCTCTAGCATCTCGAGATGCTAGAGATATTAGAAGGG-3'

Other clones with same target sequence:

(none)