Construct: shRNA TRCN0000197662

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153800.2-1039s1c1
DNA Barcode:
CCACTCAGATGTCAATAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arhgap22 (239027)
Gene Description:
Rho GTPase activating protein 22
Transcript:
RefSeq NM_153800.2 (NON-CURRENT)
Match location:
Position 1039 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197662)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTCAGATGTCAATAAGAT
Hairpin Sequence:
5'-CCGG-CCACTCAGATGTCAATAAGAT-CTCGAG-ATCTTATTGACATCTGAGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTCAGATGTCAATAAGATCTCGAGATCTTATTGACATCTGAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTCAGATGTCAATAAGATCTCGAGATCTTATTGACATCTGAGTGG-3'

Other clones with same target sequence:

(none)