Construct: shRNA TRCN0000197755

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177269.2-958s1c1
DNA Barcode:
GAATGTGGACAGATACAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atad2b (320817)
Gene Description:
ATPase family, AAA domain containing 2B
Transcript:
RefSeq NM_177269.2 (NON-CURRENT)
Match location:
Position 958 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197755)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATGTGGACAGATACAGAAT
Hairpin Sequence:
5'-CCGG-GAATGTGGACAGATACAGAAT-CTCGAG-ATTCTGTATCTGTCCACATTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATGTGGACAGATACAGAATCTCGAGATTCTGTATCTGTCCACATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATGTGGACAGATACAGAATCTCGAGATTCTGTATCTGTCCACATTC-3'

Other clones with same target sequence:

(none)