Construct: shRNA TRCN0000197773

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030026.1-988s1c1
DNA Barcode:
GAGGAGTCTCAATTATCAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mccc2 (78038)
Gene Description:
methylcrotonoyl-Coenzyme A carboxylase 2 (beta)
Transcript:
RefSeq NM_030026.1 (NON-CURRENT)
Match location:
Position 988 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197773)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGAGTCTCAATTATCAGAA
Hairpin Sequence:
5'-CCGG-GAGGAGTCTCAATTATCAGAA-CTCGAG-TTCTGATAATTGAGACTCCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGAGTCTCAATTATCAGAACTCGAGTTCTGATAATTGAGACTCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGAGTCTCAATTATCAGAACTCGAGTTCTGATAATTGAGACTCCTC-3'

Other clones with same target sequence:

  1. TRCN0000293100