Construct: shRNA TRCN0000197828

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130892.2-640s1c1
DNA Barcode:
GAACATGATGTTACCTATCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rtn4ip1 (170728)
Gene Description:
reticulon 4 interacting protein 1
Transcript:
RefSeq NM_130892.2 (NON-CURRENT)
Match location:
Position 640 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197828)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACATGATGTTACCTATCAT
Hairpin Sequence:
5'-CCGG-GAACATGATGTTACCTATCAT-CTCGAG-ATGATAGGTAACATCATGTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACATGATGTTACCTATCATCTCGAGATGATAGGTAACATCATGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACATGATGTTACCTATCATCTCGAGATGATAGGTAACATCATGTTC-3'

Other clones with same target sequence:

(none)