Construct: shRNA TRCN0000197836

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198170.2-2964s1c1
DNA Barcode:
GTTGATTGAATCATGGATCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Szt2 (230676)
Gene Description:
seizure threshold 2
Transcript:
RefSeq NM_198170.2 (NON-CURRENT)
Match location:
Position 2964 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197836)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTGATTGAATCATGGATCTA
Hairpin Sequence:
5'-CCGG-GTTGATTGAATCATGGATCTA-CTCGAG-TAGATCCATGATTCAATCAAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTGATTGAATCATGGATCTACTCGAGTAGATCCATGATTCAATCAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTGATTGAATCATGGATCTACTCGAGTAGATCCATGATTCAATCAAC-3'

Other clones with same target sequence:

(none)