Construct: shRNA TRCN0000197886
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_019458.2-974s1c1
- DNA Barcode:
- GAACCAGTTTGTGGCTTATTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Paf1 (54624)
- Gene Description:
- Paf1, RNA polymerase II complex component
- Transcript:
- RefSeq NM_019458.2 (NON-CURRENT)
- Match location:
- Position 974 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000197886)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GAACCAGTTTGTGGCTTATTT
- Hairpin Sequence:
-
5'-CCGG-GAACCAGTTTGTGGCTTATTT-CTCGAG-AAATAAGCCACAAACTGGTTC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGAACCAGTTTGTGGCTTATTTCTCGAGAAATAAGCCACAAACTGGTTCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGAACCAGTTTGTGGCTTATTTCTCGAGAAATAAGCCACAAACTGGTTC-3'
Other clones with same target sequence:
-
TRCN0000277262
