Construct: shRNA TRCN0000197903

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028197.1-2152s1c1
DNA Barcode:
GCCTAGAGTTCTAAATGTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kif1bp (72320)
Gene Description:
KIF1 binding protein
Transcript:
RefSeq NM_028197.1 (NON-CURRENT)
Match location:
Position 2152 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197903)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTAGAGTTCTAAATGTTAA
Hairpin Sequence:
5'-CCGG-GCCTAGAGTTCTAAATGTTAA-CTCGAG-TTAACATTTAGAACTCTAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTAGAGTTCTAAATGTTAACTCGAGTTAACATTTAGAACTCTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTAGAGTTCTAAATGTTAACTCGAGTTAACATTTAGAACTCTAGGC-3'

Other clones with same target sequence:

(none)