Construct: shRNA TRCN0000197927

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027482.1-484s1c1
DNA Barcode:
GTCATTATTTATGTACAGCAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
5730508B09Rik (70617)
Gene Description:
RIKEN cDNA 5730508B09 gene
Transcript:
RefSeq NM_027482.1 (NON-CURRENT)
Match location:
Position 484 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197927)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCATTATTTATGTACAGCAG
Hairpin Sequence:
5'-CCGG-GTCATTATTTATGTACAGCAG-CTCGAG-CTGCTGTACATAAATAATGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCATTATTTATGTACAGCAGCTCGAGCTGCTGTACATAAATAATGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCATTATTTATGTACAGCAGCTCGAGCTGCTGTACATAAATAATGAC-3'

Other clones with same target sequence:

(none)