Construct: shRNA TRCN0000197942

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001013608.1-851s1c1
DNA Barcode:
GTAAAGGAGTTTGCTGAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
9330134C04Rik (432766)
Gene Description:
RIKEN cDNA 9330134C04 gene
Transcript:
RefSeq NM_001013608.1 (NON-CURRENT)
Match location:
Position 851 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197942)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAAAGGAGTTTGCTGAACAA
Hairpin Sequence:
5'-CCGG-GTAAAGGAGTTTGCTGAACAA-CTCGAG-TTGTTCAGCAAACTCCTTTAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAAAGGAGTTTGCTGAACAACTCGAGTTGTTCAGCAAACTCCTTTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAAAGGAGTTTGCTGAACAACTCGAGTTGTTCAGCAAACTCCTTTAC-3'

Other clones with same target sequence:

(none)