Construct: shRNA TRCN0000197956

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178896.2-924s1c1
DNA Barcode:
GACGAATTTGTGGAATGGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dcun1d4 (100737)
Gene Description:
DCN1, defective in cullin neddylation 1, domain containing 4 (S. cerevisiae)
Transcript:
RefSeq NM_178896.2 (NON-CURRENT)
Match location:
Position 924 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197956)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACGAATTTGTGGAATGGTAT
Hairpin Sequence:
5'-CCGG-GACGAATTTGTGGAATGGTAT-CTCGAG-ATACCATTCCACAAATTCGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACGAATTTGTGGAATGGTATCTCGAGATACCATTCCACAAATTCGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACGAATTTGTGGAATGGTATCTCGAGATACCATTCCACAAATTCGTC-3'

Other clones with same target sequence:

(none)