Construct: shRNA TRCN0000197957

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198294.2-6328s1c1
DNA Barcode:
GCTGGATATAAATGTGACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tanc1 (66860)
Gene Description:
tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1
Transcript:
RefSeq NM_198294.2 (NON-CURRENT)
Match location:
Position 6328 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197957)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGATATAAATGTGACTTT
Hairpin Sequence:
5'-CCGG-GCTGGATATAAATGTGACTTT-CTCGAG-AAAGTCACATTTATATCCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGATATAAATGTGACTTTCTCGAGAAAGTCACATTTATATCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGATATAAATGTGACTTTCTCGAGAAAGTCACATTTATATCCAGC-3'

Other clones with same target sequence:

(none)