Construct: shRNA TRCN0000197990

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012011.1-333s1c1
DNA Barcode:
CCTGATGAGTTTCCTTCAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eif2s3y (26908)
Gene Description:
eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked
Transcript:
RefSeq NM_012011.1 (CURRENT)
Match location:
Position 333 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197990)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGATGAGTTTCCTTCAGAT
Hairpin Sequence:
5'-CCGG-CCTGATGAGTTTCCTTCAGAT-CTCGAG-ATCTGAAGGAAACTCATCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGATGAGTTTCCTTCAGATCTCGAGATCTGAAGGAAACTCATCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGATGAGTTTCCTTCAGATCTCGAGATCTGAAGGAAACTCATCAGG-3'

Other clones with same target sequence:

(none)