Construct: shRNA TRCN0000197994

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026877.1-1169s1c1
DNA Barcode:
CAGATGAAGGAGAAACTGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aspscr1 (68938)
Gene Description:
alveolar soft part sarcoma chromosome region, candidate 1 (human)
Transcript:
RefSeq NM_026877.1 (NON-CURRENT)
Match location:
Position 1169 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000197994)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGATGAAGGAGAAACTGGAA
Hairpin Sequence:
5'-CCGG-CAGATGAAGGAGAAACTGGAA-CTCGAG-TTCCAGTTTCTCCTTCATCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGATGAAGGAGAAACTGGAACTCGAGTTCCAGTTTCTCCTTCATCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGATGAAGGAGAAACTGGAACTCGAGTTCCAGTTTCTCCTTCATCTG-3'

Other clones with same target sequence:

(none)