Construct: shRNA TRCN0000198014

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053093.1-823s1c1
DNA Barcode:
CAACGACGAGTTAGCAGTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tac4 (93670)
Gene Description:
tachykinin 4
Transcript:
RefSeq NM_053093.1 (CURRENT)
Match location:
Position 823 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198014)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACGACGAGTTAGCAGTAAT
Hairpin Sequence:
5'-CCGG-CAACGACGAGTTAGCAGTAAT-CTCGAG-ATTACTGCTAACTCGTCGTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACGACGAGTTAGCAGTAATCTCGAGATTACTGCTAACTCGTCGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACGACGAGTTAGCAGTAATCTCGAGATTACTGCTAACTCGTCGTTG-3'

Other clones with same target sequence:

(none)