Construct: shRNA TRCN0000198021

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026222.1-2867s1c1
DNA Barcode:
CAGCTAGTAGTGGCAGTAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccdc39 (51938)
Gene Description:
coiled-coil domain containing 39
Transcript:
RefSeq NM_026222.1 (NON-CURRENT)
Match location:
Position 2867 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198021)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCTAGTAGTGGCAGTAATT
Hairpin Sequence:
5'-CCGG-CAGCTAGTAGTGGCAGTAATT-CTCGAG-AATTACTGCCACTACTAGCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCTAGTAGTGGCAGTAATTCTCGAGAATTACTGCCACTACTAGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCTAGTAGTGGCAGTAATTCTCGAGAATTACTGCCACTACTAGCTG-3'

Other clones with same target sequence:

(none)